1. Introduction: Biotechnology and Biochemical Engineering

2. Enzyme Kinetics: Kinetics, bioreactor design, inhibition, etc.

3. Immobilized Enzyme: Immobilization techniques and effect of mass transfer resistance

4. Industrial Applications of Enzymes: Carbohydrates, starch conversion, and cellulose conversion

5. Cell Cultivations: Microbial, animal, and plant cell cultivations, cell growth measurement, and cell immobilization

6. Cell Kinetics and Fermenter Design: Growth Cycle, Kinetics, batch and continuous stirred-tank fermenter, multiple fermenters in series, cell recycling, alternative fermenters, and structured kinetic models

7. Genetic Engineering: DNA and RNA, cloning of genes, stability of recombinant cells, genetic engineering of plant cells

8. Sterilization: Sterilization methods, thermal death kinetics, design criterion, batch and continuous sterilization, and air sterilization

9. Agitation and Aeration: Basic mass-transfer concepts, measurement techniques, mass-transfer coefficient, interfacial area, gas hold-up, power consumption, oxygen absorption rate, volumetric mass transfer coefficient, scale-up, and shear sensitive mixing

10 Downstream Processing: Filtration, centrifugation, cell rupture, extraction, adsorption, precipitation, chromatography, electrophoresis, membrane separation.

Total 350 pages, formatted for easy viewing and printing.